You are here:HOME>News>Export>Molecular Diagnostic Enzyme Raw Materials - Your One-Stop Service at Seebio

Molecular Diagnostic Enzyme Raw Materials - Your One-Stop Service at Seebio

Time:2023-12-05 Hits:184

Enzyme Raw Materials for Molecular Diagnostics

In Vitro Diagnostics
Molecular diagnostic technology refers to the use of DNA and RNA as diagnostic materials. Through molecular biology techniques, it detects the presence, defects, or abnormal expression of genes. This allows for the diagnosis of the individual's health status and diseases. The basic principle involves examining whether there are structural changes in DNA or RNA, the quantity of these molecules, and whether their expression functions are abnormal. This helps determine whether the individual has any abnormal changes at the genetic level, which is of crucial importance for the prevention, prediction, diagnosis, and prognosis of diseases. In simple terms, all methodological techniques based on the molecular biology level are considered part of molecular diagnostic technology, such as PCR technology, gene sequencing, and more.
Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific nucleotide segments. It can be viewed as a special nucleic acid replication that occurs outside a living organism. PCR is characterized by its ability to significantly amplify minute amounts of nucleic acids, making them easily identifiable and distinguishable.
PCR Technology
PCR can amplify target DNA segments more than a million times. The principle involves using DNA polymerase to catalyze the process, using the parent DNA strand as a template and specific primers as extension starting points. Through denaturation, annealing, extension, and other steps, PCR replicates the process of generating a complementary DNA strand from the parent DNA template outside the living organism.
The standard PCR process consists of three steps:
Denaturation: Utilizing high temperature to separate the DNA double strands. The hydrogen bonds between the DNA double strands are disrupted at elevated temperatures (93-98°C).
Annealing: After the DNA double strands are separated, the temperature is lowered, allowing primers to bind to the single-stranded DNA.
Extension: DNA polymerase starts synthesizing the complementary strand along the DNA chain from the primer-binding site during the temperature drop. Once extension is complete, one cycle is finished, and the number of DNA fragments doubles.
Repeating these three steps 25-35 times exponentially increases the number of DNA fragments.
PCR technology has evolved through continuous technological advancements, including conventional PCR, quantitative fluorescent PCR, digital PCR, and more.
Molecular diagnostic technology has progressed through DNA molecular hybridization, PCR, and biochips, and is now entering the era of gene sequencing. Due to its widespread applications, this industry has experienced rapid global development. In the entire molecular diagnostic industry, as an upstream sector, the product quality and stability of diagnostic materials directly impact the development of downstream products. To better serve the global molecular diagnostic market.
PCR Product List:
series
type
Item number
name
Size
High Security Real PCR Series
high fidelity DNA polymerase
Gold High-Fidelity DNA Polymerase
High-fidelity DNA polymerase
10U、100U、500U、1KU
High-Fidelity DNA Polymerase
High-fidelity DNA polymerase
10U、100U、500U、1KU
high fidelity PCR master mix
DAA0175A
2×Seebio® Gold PCR Master Mix
High fidelity enzyme master mix
1ml
2×PCR Master Mix
High fidelity enzyme master mix
100μl、1ml、5×1ml、25×1ml
5×PCR Master Mix
High-fidelity enzyme master mix (for multiplex PCR)
1ml、5×1ml、25×1ml、500ml
2×PCR Master Mix
High-fidelity enzyme master mix (for multiplex PCR)
1ml、25ml、50ml、250ml、500ml
2×Gold PCR Master Mix
High fidelity enzyme master mix
100μl、1ml、5×1ml
Hot Start PCR
Taq DNA polymerase
Soap® sTaq DNA Polymerase sTaq DNA Polymerase (without dNTPs)
200And、1KU、5KU
Soap® sTaq DNA Polymerase sTaq DNA polymerase (contains dNTP)
200And、1KU、5KU
Soap® Enhances sTaq DNA Polymerase Soap® Enhan sTaq DNA Polymerase (contains dNTPs)
200And、1KU、5KU
other DNA polymerase
Soap® COD DNA Polymerase
Soap® KOD DNA polymerase (without dNTPs)
200U、1KU
Soap® COD DNA Polymerase
Soap® KOD DNA polymerase (contains dNTPs)
120U、200U、1KU
Soap® sPFU DNA Polymerase
Soap® sPfu DNA polymerase (without dNTPs)
200U、1KU
Soap® sPFU DNA Polymerase
Soap® sPfu DNA polymerase (contains dNTPs)
200U、1KU
DAA0113A
Long Taq DNA Polymerase
long fragment DNA polymerase
5×125U、5KU
Hot Start
Taq DN polymerase
DAA0124A
Soap® HotStart Taq DNA Polymerase
Antibody method hot start Taq enzyme
500IN
HotStart Taq DNA Polymerase
Ligand method hot start DNA polymerase
250U、4×250U
Hotstart C-Taq DNA Polymerase
Antibody method hot start C-Taq DNA polymerase
100U、500U、3KU
DHS J-Taq DNA Polymerase
Antibody method hot start J-Taq DNA polymerase
100IN
DHS B-Taq DNA Polymerase
Antibody method hot start B-Taq DNA polymerase
100IN
Hotstart K-Taq DNA Polymerase
Antibody method hot start K-Taq DNA polymerase
-
Hotstart J-Taq DNA Polymerase
Antibody method hot start J-Taq DNA polymerase
-
Hotstart B-Taq DNA Polymerase
Antibody method hot start B-Taq DNA polymerase
100IN
hot start
Taq enzyme
blocking antibody
EKN0168A
Anti-Taq DNA Polymerase Antibody(5U/UL)
500IN
Multiplex PCR
multiplex PCR
master mix
DAA0854A
5X Seebio® C1P1 Multiplex PCR Master Mix
5X C1P1 Multiplex PCR Master Mix
1ml
RT-PCR
reverse transcriptase
Soap® Reverse Transcriptase Soap® reverse transcriptase
2KU, 10KU, 4×10KU
RNase inhibitor
RNase Inhibitor RNase inhibitor
10KU
cDNA one  chain synthesis
Soap® 1st Strand cDNA Synthesis Kit Soap® Dedicated kit for cDNA one-strand synthesis
100T、500T、2000T
other
isothermal amplification
DAA0792A
Bst II DNA Polymerase
Bst II DNA polymerase
1600IN
BST DNA Polymerase  Large Fragment
BST DNA polymerase large fragment
800U、8KU
UDG enzyme
DAA0230A
Uracil DNA Glycosylase(UDG),heat-labile
Thermal UDG
100IN
DAA0232A
Uracil DNA Glycosylase(UDG)
UDG enzyme
100IN
PCR
master mix
Soap®  sTaq Premix sTaq PCR Mix master mix (no dye)
2×500μl
Soap® sTaq Premix  sTaq PCR Mix master mix (contains xylonitrile FF and tartrazine)
2×500μl
Soap® sTaq Premix+  sTaq PCR Mix master mix (contains SYBR Green II)
2×500μl
dNTP mixture
Soap® dNTP Mixture dNTP Mixture
800μl
other molecular diagnosis Reagent
gene editing Enzymes for sequencing
DAA0332B
E.coli DNA ligase DNA ligase
300U、1200U
Taq DNA Ligase Taq DNA Ligase
1KU, 2KU, 10KU
T4 RNA Ligase 1 T4 RNA Ligase1
1KU, 2KU, 10KU
Seebio® T7 RNA Polymerase
Seebio® T7 RNA Polymerase
5KU、25KU、50KU
Seebio® KAPA HiFi Hot  Start for NGS  Seebio® KAPA HiFi Takaho Mankei
100U、250U
Seebio® Tn5 Transposase
Seebio® Tn5 transposase
800pmol、4000pmol
other
EBY0027A
Proteinase K Proteinase K (classic)
100mg、500mg
Proteinase K Proteinase K (water-soluble)
100mg、500mg
Proteinase K Proteinase K (glycerol-soluble)
100mg、500mg
Ribonuclease A Ribonuclease A (RNase A)
25mg、100mg
DENARASE versatile nuclease
100KU、5MU
Terminal Deoxynucleotidyl Transferase
TDT terminal transferase
500IN
Exonuclease I (E. coli) Type I exonuclease
1500And、15KU
Alkaline Phosphatase (30 U/ml), Calf Intestine (CIAP)
calf intestinal alkaline phosphatase
1KU
Shrimp Alkaline Phosphatase (SAP) (1 U/mL)  shrimp alkaline phosphatase
250IN
Lysozyme Lysozyme
5g、10×5g
Lyticase (10U/ml) Wall-lytic enzyme
1500U、3KU、10KU
Snailase
5g、10g
Ribonuclease A (RNase A)  from bovine pancreas
100mg、1g
Deoxyribonuclease I (DNase I) from bovine pancreas
DNAse I, derived from bovine pancreas
15KU、10×15KU
Deoxyribonuclease I (DNase I) from bovine pancreas
25mg、100mg、1g
Exonuclease(3'-5') exonuclease
100U、500U、2KU
Aprotinin aprotinin
5mg、50mg
Phosphatase Inhibitor Cocktail
Phosphatase inhibitor cocktail(100×,storage solution)
2ml、10×2ml、100×2ml
Leupeptin Leupeptin (ultrapure)
5mg、10×5mg
Enterokinase, Recombinant, Expressed in E. coli
E. coli expressing recombinant enterokinase (without tag)
100U、200U、500U、1KU、5KU
rTEV Protease
Recombinant tobacco etch virus protease
1KU、10KU、50KU、10×1KU
Seebio Biotech continually pioneers and innovates in the upstream raw materials field for diagnostic reagents, establishing the Seebio Life Sciences Research Laboratory. The aim is to provide high-quality molecular diagnostic raw materials to the global market, including Seebio® molecular diagnostic materials (enzymes, primers, probes, second-generation sequencing library materials, and modules), along with comprehensive project collaboration solutions. Leveraging expertise in molecular enzyme development and production, Seebio Biotech can offer a range of solutions, from technical validation to research project initiation, laboratory development, product qualification, pilot production, quality control, to product manufacturing, for clients across various sectors such as biotechnology, pharmaceuticals, precision medicine, sequencing, CRO, and more.
For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578