Hygromycin B

Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.

Assay：90%HPLC

valence：1109u/mg
melting point：160-180℃
preserving temperature：2～8℃，light should be avoided

work concentration:
prokaryote(e.g., E. coli, at 100 mg/ml); lower eukaryotes (e.g., yeast, at 200 mg/ml) and higher eukaryotes (e.g., mammalian cells in culture, 150-400 mg/ml)

Hygromycin B is soluble in water, at concentrations >50 mg/mL, and stable in aqueous solution。Solutions should be sterilized by filtration, not autoclaving。

Thermal stability:
Aqueous solutions can remain stable for at least a month from37℃ to 60℃。

Mammalian cells: 50-1000ug/mL
Plant cells: 20-200ug/mL
Bacteria: 20-200ug/mL
Fungi: 200-1000ug/mL

Your appropriate concentration should be tested experimentally!

Packing：25kg/fiber paper board drum

Application:

Hygromycin B has certain deworming activity, it has good nematode flooding effect if it is added in feed for pigs and poultry for long term, As a repellent resistant drug it can be used in chicken and pig feed.

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