Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF ,aprotinin,soybean
trypsin inhibitor and by metal chelating agents, e.g.EDTA.It is typically isolated from the pancreas of animals.Animal-derived materials
are now subject to more strigent regulations, as authorities strive to ensure safer biologics and pharmaceuticals,such as in insulin and
vaccine production.
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. Recombinant trypsin is expressed as active protease , with equivalent properties compared to native trypsin .It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines.No animal derived products are used in the fermentation,purification,or final formulation.
CAS: 9002-07-7
EC: 3.4.21.4
Source: Porcine trypsin,expressed in E.coli.
ADVANTAGES
Animal origin free:No animal-derived material is used during production.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
2) Reduce production costs while improving manufacturing progresses.
MAIN FEATURES
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Source
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E. Coli
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Purified by
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HPLC
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Format
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White lyophilized powder
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Specific activity
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≥3800 USP units/mg pro
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Purity（RP-HPLC)
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NLT 70% β-trysin, NMT 20% α-trypsin
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UNIT DEFINITION: One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
RECOMMEND USAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
STORAGE INSTRUCTIONS
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Function:
It is used to produce virus vaccine for treating mammalian cells infection.
Culturing of therapy and primary cells;
Stem cell culture;
The production of recombinant proteins.
