Synonym: RCPB01
Recombinant carboxypeptidase B (EC 3.4.17.2) is specially used for hydrolysis of terminal end alkaline amino acid(lysine, arginine, histidine) of protein C; it is also called as hydrolytic enzyme of peptidyl -L- lysine (L- arginine) and protaminase. The molecular weight is 35kD, isoelectric point is 6, the optimal pH is 7-9. Its’ activity is competitively inhibited by arginine and lysine, metal ion chelating agents such as EDTA also inhibit the enzyme activity.
Product advantage:
Not from animal source: reorganization producing, no exogenous viruses contamination, such as swine flu virus, porcine parvovirus etc.
High purity: It has no other miscellaneous enzyme activity, it has no other side reaction in enzyme reaction:, it has high yield.
High specific activity:
Electrophoresis Purity: electrophoresis identification of SDS-PAGE, 95%
Stability: It is freeze-dried powder, easy to store and transport
Qualification：
Specific activity: ≥120 u/mg pro.；≥170 u/mg pro.
Source: Recombinant Escherichia coli
Properties: White or off white freeze-dried powder
Electrophoresis and Coomassie brilliant blue 12%SDS-PAGE：The molecular weight is 35kd, a single band.
Content of other enzyme: No chymotrypsin, carboxypeptidase A, trypsin content is less than 10ppm
Protease inhibitor: there is not any other protease inhibitors including PMSF, EDTA etc.
Package: 10mg,100mg,1g,bulk
Application:
Production of recombinant insulin and its analogs
Determination of terminal end amino acid of protein C.
Other recombinant polypeptide substance production
Enzymatic synthesis of some special compound.
