Summary
Purity > 95 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU per μg protein as determined by the LAL method.
Activity Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag)(40592-V05H),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL.
Protein Construction A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Expressed Host HEK293 Cells
Molecule Mass The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
Form Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.