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ACH0012E

Guanidine thiocyanate

for molecular biology
  • CAS Number 593-84-0
  • Molecular Formula CH5N3HSCN
  • Molecular Weight 118.16
  • EC Number 209-812-1
  • Beilstein Registry Number 3563461
  • MDL number MFCD00013027
  • PubChem Substance ID 24895367
  • Function: molecular biology
  • Application: RNA isolation, DNA extraction, PCR inhibitor
  • Negotiable/25Kg/Drum
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    Summary

      Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol: chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA.

    Properties

      Purity ≥99.5%
      Solubility Clear and colorless
      UV Abs.@280nm ≤0.6 (3M in water)
      UV Abs.@300nm ≤0.2 (3M in water)
      Melting point 118~121
      pH(11.8%,water solution) 5.0~7.0
      Moisture ≤0.5%
      Ash ≤0.5%
      Cd 5ppm
      Co 5ppm
      Cr 5ppm
      Cu 5ppm
      Fe 5ppm
      Mg 5ppm
      Mn 5ppm
      Ni 5ppm
      Pb 5ppm
      DNase Not detected
      RNase Not detected
      Appearance White crystals

    Product Detail

      Application

      Guanidine thiocyanate has been used:

      • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation

      • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay

      • for DNA extraction from cerebrospinal fluid (CSF)