Summary
Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol: chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA.
Properties
Purity | ≥99.5% |
Solubility | Clear and colorless |
UV Abs.@280nm | ≤0.6 (3M in water) |
UV Abs.@300nm | ≤0.2 (3M in water) |
Melting point | 118~121℃ |
pH(11.8%,water solution) | 5.0~7.0 |
Moisture | ≤0.5% |
Ash | ≤0.5% |
Cd | ≤5ppm |
Co | ≤5ppm |
Cr | ≤5ppm |
Cu | ≤5ppm |
Fe | ≤5ppm |
Mg | ≤5ppm |
Mn | ≤5ppm |
Ni | ≤5ppm |
Pb | ≤5ppm |
DNase | Not detected |
RNase | Not detected |
Appearance | White crystals |
Product Detail
Application
Guanidine thiocyanate has been used:
• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
• for DNA extraction from cerebrospinal fluid (CSF)