Summary
In the recombinant human trypsin, the sequence and the nature of amino acid is completely same with human trypsin. Trypsin is a kind of endopeptidase, it can be used to shear peptide bond of lysine and C end of arginine, thus split the macromolecular protein into small peptide.
It is gene engineering produced, non animal source, no virus pollution, non hybrid enzyme pollution
Properties
Qualification:
|
Items |
Content |
|
Sources |
recombinant Escherichia coli |
|
Purification |
HPLC |
|
Properties |
white or off white freeze-dried powder |
|
Electrophoresis and Coomassie brilliant blue staining 12%SDS-PAGE |
Non reduced SDS-PAGE protein electrophoresis, the molecular weight is24kD, a single main band. |
|
Specific activity |
≥2500 USPu/mgpro. ≥3800USPu/mgpro |
|
Other enzyme content |
not polluted by chymotrypsin and carboxypeptidase A and activity |
|
It does not contain any protease inhibitors |
It doesn’t contain any PMSF, EDTA and other protease inhibitors |
Activity unit：25℃，pH7.6，reaction system 3.0ml(1cm light path)，each minute emphasis BAEE make absorption value below 253nm increase 0.003 , it is a USP unit.
Packing:10mg,100mg,1g, bulk
Product Detail
Trypsin is widely used in various biotechnological processes, such as:
1) cell separation of various cell cultureed tissue
2) degradation of modified protein
3) protein enzymolysis and sequencing
4) cells treatment of cancer, etc.