Summary
Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome.
Price list
Size
Price (USD)
100MG
379
250MG
705
1G
1900
10G
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100G
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Properties
| form | lyophilized powder |
| purified by | ion-exchange chromatography |
| solubility | H2O: soluble50 mg/mL |
| antibiotic activity spectrum | viruses |
| storage temp. | 2-8°C |
Product Detail
Hygromycin B is an aminoglycoside antibiotic substance naturally produced by Streptomyces hygroscopicus, which also shows anthelmintic activity. Hygromycin B has been shown to effectively inhibit translocation of mRNA and tRNAs on the ribosome in both bacteria and eukaryotes and also has a subtle effect on decoding fidelity as it slightly increases aminoacyl tRNA (A-site tRNA) affinity. Suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.