Soluble Growth Stimulating Expression Gene 2 Protein (ST2) - Magnetic Particle Chemiluminescence Method (Acridinium Ester)/Fluorescence Immunochromato
Time：2024-02-20 Hits：222
Project Background
Growth stimulation expressed gene 2 (ST2), also known as interleukin 1 receptor-like 1 (IL1RL-1), belongs to the interleukin 1 receptor family. It is a protein in the heart that responds to disease or injury, specifically expressed by cardiac fibroblasts. This protein is directly involved in cardiac fibrosis or cardiac remodeling. The normal value is <35ng>35ng/ml indicates a deviation from normal, prompting further diagnosis by a physician based on clinical symptoms and additional test indicators.
ST2 serves as a pivotal indicator for assessing risk classification and prognosis in patients with acute and chronic heart failure. It reflects the degree of myocardial fibrosis, unaffected by factors such as age, gender, race, BMI, or renal function. ST2 is a singular threshold marker for heart failure, and its concentration correlates with the severity of the condition. It aids in observing the efficacy of antimicrobial therapy and myocardial fibrosis drugs in patients. The combined testing of ST2 and NT-proBNP enhances the prediction of cumulative risk in acute heart failure and improves the ability to predict death in heart failure patients.
"2017 American ACC/AHA/HFSA Heart Failure Management Guidelines" and the "Chinese Heart Failure Diagnosis and Treatment Guidelines 2018" jointly recommend ST2 for risk stratification and prognosis assessment in heart failure patients. The "Chinese Expert Consensus on Heart Failure Biomarkers 2020" highlights the crucial auxiliary role of sST2 in diagnosing acute decompensated heart failure, boasting a high negative predictive value. It also serves as a reference for prognostic assessment in heart failure patients, with dynamic increases indicating a poor prognosis.
Performance
Chemiluminescence (Acridinium Ester) Platform:
Combination 1: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100E) coated carboxyl magnetic beads + Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100F) labeled acridinium ester;
Linear range: 3.1ng/mL-200ng/mL;
Combination 2: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100E) coated carboxyl magnetic beads + Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100C) labeled acridinium ester;
Linear range: 3.1ng/mL-200ng/mL;
Chemiluminescence (Acridinium Ester) Platform Calibration Curve
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Combination 1: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100C) labeled fluorescent microspheres + Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100E) for scratching;
Linear range: 3.1ng/mL-200ng/mL.
Combination 2: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100C) labeled fluorescent microspheres + Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Product No.: EKY0100F) to draw membrane;
Linear range: 3.1ng/mL-200ng/mL.
Fluorescence Immunochromatography Platform Calibration Curve
Clinical Relevance
Chemiluminescence (Acridinium Ester) Platform:
The clinical correlation coefficient (R2) between two sets of ST2 antibodies from the Xibao Bioluminescence Platform and the reagent provided by Zhonghan Shengtai ST2 reagent (Fluorescence Immunochromatography) exceeded 0.95, indicating a strong correlation.
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
The clinical correlation coefficient (R2) for the two sets of ST2 antibody pairs from the Xibao Biofluorescence Platform and the reagent provided by Zhonghan Shengtai ST2 reagent (Fluorescence Immunochromatography) was >0.95, demonstrating a robust correlation.
Test Program
Chemiluminescence (Acridinium Ester) Platform:
Combination 1
Antigen to be tested: ST2 antigen (Cat. No.: EDD0746A/EDD0746B), sample/calibrator adding volume: 20ul;
Reagent 1: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Cat. No.: EKY0100E) coated-magnetic bead dilution, dosage 50ul/test;
Reagent 2: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Cat. No.: EKY0100F) labeled-acridinate dilution, dosage 50ul/test;
Combination 2
Antigen to be tested: ST2 antigen (Cat. No.: EDD0746A/EDD0746B), sample/calibrator adding volume: 20ul;
Reagent 1: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Cat. No.: EKY0100E) coated-magnetic bead dilution, dosage 50ul/test;
Reagent 2: Soluble Growth Stimulating Expression Gene 2 Protein (ST2) Antibody (Cat. No.: EKY0100C) labeled-acridinate dilution, dosage 50ul/test;
Luminescence instrument used in the test: Cosmai SMART 500S; a chemiluminescence instrument compatible with various acridinium ester systems (such as Sichuan Mike, Shenzhen Yahuilong, Shandong Kanghua Bio, Getein Bio, Nanjing Norman, etc.).
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Wet Method (ST2 Antibody-labeled Fluorescent Microspheres Diluted in Liquid)
Antigen to be tested: ST2 antigen (Cat. No.: EDD0746A/EDD0746B), sample/calibrator adding volume: 5ul;
Reaction conditions: mix sample + fluorescent solution, load sample, reaction time (10min-15min);
Dry Type (ST2 Antibody-labeled Fluorescent Microspheres Sprayed on the Release Pad)
Antigen to be tested: ST2 antigen (Cat. No.: EDD0746A/EDD0746B), sample/calibrator adding volume: 80ul;
Reaction conditions: mix sample + diluent, load sample, reaction time (10min-15min);
Fluorescence instrument used for testing: Hemai FIC-S100; an instrument compatible with various fluorescence systems (excitation wavelength 365nm, emission wavelength 615nm) (such as Guangzhou Labsim, etc.).
Product Information
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Product
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Item Number
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Recommended pairing
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Soluble growth-stimulating expressed gene 2 protein (ST2) antibody
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EKY0100E
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Soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100E) coated carboxyl magnetic beads + soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100F) labeled acridinium ester（Luminous Pairing Set1）
Soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100E) coated carboxyl magnetic beads + soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100C) labeled acridinium ester（Luminous Pairing Set2）
Soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100C) labeled fluorescent microspheres + soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100E) streaking（Fluorescence pair combination1）
Soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100C) labeled fluorescent microspheres + soluble growth-stimulating gene 2 protein (ST2) antibody (Cat. No.: EKY0100F) streaking（Fluorescence pair combination2）
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Soluble growth-stimulating expressed gene 2 protein (ST2) antibody
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EKY0100F
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Soluble growth-stimulating expressed gene 2 protein (ST2) antibody
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EKY0100C
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Soluble growth-stimulating expressed gene 2 protein (ST2) antigen
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EDD0746A
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Soluble growth-stimulating expressed gene 2 protein (ST2) antigen-His Tag
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EDD0746B
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ST2 monoclonal antibody (EKY0100E)-carboxy magnetic bead concentrate
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DKY1315A
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ST2 monoclonal antibody (EKY0100F)-acridinate concentrate
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DKY1315B
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ST2 monoclonal antibody (EKY0100C)-acridinate concentrate
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DKY1315C
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ST2 monoclonal antibody (EKY0100C) labeled fluorescent microsphere concentrate
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DKY1315D
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Carboxy magnetic beads (superparamagnetic), 1um
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DEG0091B-1-100mg
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Carboxy magnetic beads (superparamagnetic), 3um
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DEG0091A-3-100mg
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Time-resolved fluorescent microspheres, carboxyl (200nm）
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AEG0786B
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