Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Detection Raw Material Solution
Time：2024-02-19 Hits：233
Lp-PLA2 Detection
Project Background
Lipoprotein-associated phospholipase A2, abbreviated as Lp-PLA2 and also known as platelet-activating factor acetyl hydrolase, is comprised of 441 amino acids with a molecular weight of 45 kDa. It plays a direct role in the vascular inflammatory response leading to atherosclerosis and serves as a novel independent marker for vascular endothelial inflammatory response.
Seebio Biotech offers raw materials for various methods of detecting lipoprotein-associated phospholipase A2, including the Lp-PLA2 substrate (1-myristoyl-2-(4-nitrophenylsuccinyl)-sn-glycerol- in the rate method 3-Phosphocholine, 14:0 NPS PC), Lp-PLA2 antigen, and antibody in immunoassay, among others.
Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Assay Method
The level of Lp-PLA2 can be assessed by measuring the quality and activity of serum (plasma) Lp-PLA2, primarily using various immunological methods such as enzyme-linked immunoassay, chemiluminescence, immunochromatography, immunofluorescence, latex enhancement, etc. Enzyme quality is determined through the rate method, measuring the enzyme's activity through hydrolysis substrate method.
Rate Method (Hydrolysis Substrate Method)
In the rate method, LP-PLA2 in the sample hydrolyzes the Sn-2 position of the substrate 1-myristoyl-2-(4-nitrophenylsuccinyl)-sn-glycero-3-phosphocholine, generating a colored product, 4-p-Nitrophenol. This product exhibits an absorption peak at 405 nm, and the Lp-PLA2 activity is calculated based on the change rate of its absorbance.
Double-Antibody Sandwich Method (Using Chemiluminescence as an Example)
This method involves labeling magnetic particles and horseradish peroxidase (HRP) with two monoclonal antibodies that recognize Lp-PLA2 antigen. After mixing with the antigen, magnetic beads coated with anti-Lp-PLA2 antibodies and HRP enzyme form a double-antibody sandwich complex. After incubation, washing, and other processes, it emits light under the action of the excitation solution (luminol-hydrogen peroxide system). The relative luminescence intensity (RLU) correlates positively with the Lp-PLA2 concentration in the sample.
Immunoenhanced Turbidimetry
In this method, LP-PLA2 in the sample reacts with sensitized latex particles of mouse anti-human LP-PLA2 monoclonal antibody, causing agglutination under the action of the accelerator polyethylene glycol, increasing turbidity. The change in absorbance at a wavelength of 546 nm is directly proportional to the LP-PLA2 content in the sample.
Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Key Raw Materials
Key Raw Materials for Rate Method
Material Code: AZE0142B
CAS#: 273931-53-6
Molecular Formula: C32H53N2O12P
Molecular Weight: 688.74
Key Raw Materials for Immunoenhanced Turbidimetry
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Item number
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Product
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Pairing information
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Recommended platform
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Lp-PLA2 protein
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ELISA, LETIA
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Mouse anti-human Lp-PLA2 monoclonal antibody
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LETIA
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Mouse anti-human Lp-PLA2 monoclonal antibody
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capture
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CLIA
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Mouse anti-human Lp-PLA2 monoclonal antibody
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mark
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Mouse anti-human Lp-PLA2 monoclonal antibody
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capture
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LFIA
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Mouse anti-human Lp-PLA2 monoclonal antibody
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mark
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