Raw and Auxiliary Materials for Triglyceride (TG) Detection Kit
Time：2024-01-31 Hits：286
Triglyceride (TG) is a neutral fat and non-polar substance, constituting a fat molecule formed from long-chain fatty acids and glycerol. This energy-rich substance is abundant in the body, providing the necessary energy for muscle movement upon decomposition. Serum triglyceride levels are influenced by factors such as diet, age, and gender.
Triglycerides play a crucial role in blood lipid examination, serving as an important indicator. Elevated triglyceride levels are commonly associated with conditions like diabetes, hypothyroidism, nephrotic syndrome, and atherosclerosis. Conversely, reduced triglyceride levels are observed in hyperthyroidism, diminished adrenal cortex function, and severe liver dysfunction.
The widely utilized method for detecting triglycerides (TG) is the GPO-PAP method. Seebio Biotech offers raw materials and excipients essential for triglyceride (TG) detection kits. For inquiries, please contact us at service@seebio.cn or call us at +86 21 58183719, or reach out via WeChat at +86 158 0195 7578.
Principle of Triglyceride (TG) Detection
In the triglyceride (TG) detection process, lipoprotein lipase (LPL) catalyzes the hydrolysis of triglycerides in the sample into glycerol and free fatty acids. Glycerol, in conjunction with adenosine triphosphate (ATP), generates glycerol 3-phosphate under the influence of glycerol kinase (GK). This compound reacts with oxygen through the action of glycerol phosphate oxidase (GPO), resulting in the production of hydrogen peroxide and dihydroxyacetone phosphate.
Hydrogen peroxide then undergoes a reaction with aniline chromogens and 4-aminoantipyrine, catalyzed by peroxidase (POD), generating water and quinoneimine pigments. The quantity of quinoneimine pigment produced is directly proportional to the triglyceride content in the sample. Measurement of the pigment generated at a specific wavelength allows for the calculation of triglyceride concentration in the sample.
Reaction Formula:
Raw and Auxiliary Materials for Triglyceride (TG) Detection Kit
|
Item Number
|
Product
|
Abbreviation
|
CAS
|
Level
|
|
Glycerol kinase
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GK/GKZ
|
9030-66-4
|
>180U/mg
|
|
|
Lipoprotein lipase
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LPL
|
|
|
|
|
Horseradish peroxidase
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HRP
|
|
150U/mg; RZ>2.0
|
|
|
L-α-Glycerophosphate oxidase
|
|
9046-28-0
|
≥20U/mg solid
|
|
|
DCE0144A
|
L-α-Glycerophosphate oxidase
|
GPO
|
|
Grade III 15U/mg-solid or more
|
|
Lipoprotein lipase
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LPL
|
9001-62-1
|
|
|
|
Adenosine 5'-triphosphate
|
ATP
|
56-65-5
|
|
|
|
Adenosine 5'-triphosphate disodium salt hydrate
|
ATP-2Na
|
34369-07-8
|
99%
|
|
|
Bovine serum albumin (fifth fraction)
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BSA
|
9048-46-8
|
Biotechnology Grade
|
|
|
Bovine serum albumin (fraction 5, protease-free)
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BSA
|
9048-46-8
|
Biotechnology Grade
|
|
|
4-Aminoantipyrine
|
4-AAP
|
1983/7/8
|
98.50%
|
|
|
KroVin 300 preservative
|
|
|
BR
|
|
|
Tris(hydroxymethyl)aminomethane
|
Tris base
|
77-86-1
|
Ultra Pure Grade
|
|
|
Tris(hydroxymethyl)aminomethane hydrochloride
|
Tris-HCl
|
1185-53-1
|
High Purity Grade
|
|
|
3,5-Dichloro-2-hydroxybenzenesulfonic acid sodium salt
|
DHBS-Na
|
54970-72-8
|
99%
|
|
|
3-[(3-Cholamidopropyl)dimethylammonium]-1-propanesulfonic acid
|
CHAPS
|
75621-03-3
|
≥98%
|
|
|
N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt
|
DIRECT/EHSPT
|
82692-93-1
|
(98.5%)Ultra Pure
|
|
|
Piperazine-N,N'-bis(2-ethylsulfonic acid)disodium salt
|
PIPES-2Na
|
76836-02-7
|
≥98%
|
|
|
Piperazine-1,4-diethanesulfonic acid
|
PIPES（Free Acid）
|
5625-37-6
|
≥99%
|
|
|
N-Ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt
|
TOPS
|
40567-80-4
|
>99%
|
|
|
Triglycerides (calibrator）
|
TG
|
|
|
|
|
Triglycerides (quality control product）
|
TG
|
|
|
|
|
4-Hydroxybenzoic acid
|
|
99-96-7
|
Reagent grade
|
|
|
Potassium hexacyanoferrate(II) trihyrate
|
|
14459-95-1
|
|
|